Long read club is part of a recently funded Wellcome Trust Technology Development Award. Our overall goal is to help the genomics community achieve finished quality, complete reference quality genomes routinely and at scale.
The first rule of long read club is … sign-up for email notifications to be informed about upcoming online events!.
Our YouTube channel is at:
Episode 1: What is Long Read Club?
Episode 2: DNA extraction with Jill Herschleb, 10X Genomics
Episode 3: Whole human genome assemblies with Adam Phillippy, NHGRI
Episode 4: The Flye assembler with Mikhail Kolmogorov and Jeffrey Yuan, UCSD
Episode 5: Introducing MorphoSeq: high accuracy long reads from short read platforms, Aaron Darling, Longas Technologies
Head over to our Protocols.io group at:
This page contains links to examplar or notable long-read datasets:
The nanopore human WGS consortium are curating a regularly-updated NA12878 human cell line data release from nanopore data. More information on their Github:
At AGBT 2019, the T2T consortium released an extensive dataset for the haploid human CHM13 cell line including 50X ultra-long nanopore coverage, 10X Genomics data, BioNano data. This is complimented by existing PacBio data. The goal of the consortium is to complete a telomere-to-telomere contiguous finished assembly of a haploid human genome.
Find out more at their website: https://sites.google.com/ucsc.edu/t2tworkinggroup/home Data release available via GitHub: https://github.com/nanopore-wgs-consortium/CHM13
As part of protocol development, we have generated extensive ultra-long read data for E. coli K-12 MG1655 which is released here in case it is useful for development (e.g. of assembly software).